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1.
Journal of Shanghai Jiaotong University(Medical Science) ; (12): 445-449, 2018.
Article in Chinese | WPRIM | ID: wpr-695687

ABSTRACT

Spermatogenesis originates from spermatogonial stem cells (SSCs).SSCs can continually renew and eventually differentiate into spermatozoa under control of various growth factors,microenvironments and self-signaling.The molecules involved in SSCs self-renewal include glial cell-derived nerve growth factor,fibroblast growth factor and downstream signaling pathways,as well as the transcription factors and the epigenetic regulators.Molecules related to SSCs differentiation include retinoic acid,a variety of transcription factors and the eptigenetic regulatory factors.The research on the mechanism of SSCs self-renewal and differentiation is of great significance for the understanding of spermatogenesis and the diagnosis and treatment of male infertility.This review summarized the exogenous factors,transcription factors,and epigenetic regulators that are involved in the regulation of SSCs self-renewal and differentiation in recent years.

2.
National Journal of Andrology ; (12): 208-213, 2015.
Article in Chinese | WPRIM | ID: wpr-319518

ABSTRACT

<p><b>OBJECTIVE</b>To isolate, identify and culture human spermatogonial stem cells (SSC) and then obtain purified and enriched human SSCs for research and application.</p><p><b>METHODS</b>We detected the expression of CD90 in the human testis using the immunofluorescence technique and isolated human testicular spermatogenic cells by two-step enzymatic digestion, followed by differential plating and magnetic-activated cell sorting (MACS) with CD90 as an SSC marker. Then we identified the isolated CD90-positive spermatogenic cells by RT-PCR and immunocytochemistry, and meanwhile cocultured them with Sertoli cells in SG medium in vitro.</p><p><b>RESULTS</b>The isolated CD90-positive cells showed a relatively homogeneous characteristic in size and morphology and expressed the genes specific for human SSCs, with high expressions (90.5%) of GFRA1, GPR125, and UCHL1. After coculture with Sertoli cells in the SG medium for 2 weeks, the isolated CD90-positive cells maintained a good activity.</p><p><b>CONCLUSION</b>CD90 can be regarded as a speci- fic marker for human SSCs and used to obtain highly enriched human SSCs by differential plating and MACS. Furthermore, the isolated human SSCs can be cultured in SG medium in vitro.</p>


Subject(s)
Humans , Male , Adult Stem Cells , Cell Biology , Biomarkers , Metabolism , Cell Separation , Methods , Cell Shape , Cell Size , Coculture Techniques , Glial Cell Line-Derived Neurotrophic Factor Receptors , Metabolism , Immunohistochemistry , Receptors, G-Protein-Coupled , Metabolism , Sertoli Cells , Spermatogonia , Cell Biology , Testis , Metabolism , Thy-1 Antigens , Metabolism , Ubiquitin Thiolesterase , Metabolism
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